Sectioning refractory woods for anatomical studies
Dept. of Agriculture, Forest Service, Forest Products Laboratory, 1977 - House & Home - 9 pages
Describes a new technique for softening wood, using a 4 percent solution of ethylenediamine; a shortcut method for removing silica and crystalline materials with hydrofluoric acid; a method for rapidly neutralizing wood blocks which have been treated with hydrofluoric acid; and a method for maintaining the flatness of sections through staining, dehydration, and clearing. Methods described are applicable to wood with a specific gravity in excess of 0.75. (Author).
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16 hours aline the transverse anatomical study applied approximately 2 centimeters beaker blocks in water bundles cell wall clamping clips code number Commercial HF cover glass curled or rolled dense woods distilled water ETD solution Ethylenediamine final vacuum floaters give the specific grain direction gravity of less heated high-quality transverse sections hold-down hot plate knife edge less than 1.00 measure approximately microtome knife millimeters moisture content nearest 0.1 one-half hour paper towel percent solution period permeability placed procedure produce satisfactory sections radial and tangential radial surface Reagents remove the blocks Sapotaceae saturated solution sectioning block sectioning of dense silica sink in water Sinkers skip slide smooth and aline sodium bicarbonate softened solution of ETD solution of sodium solution strength specific gravity value specimen length staining submerged length tangential surface temperature tongs transverse surface treated treatment trimming volume waterproof ink weight width wood blocks wood collection specimens wood rays wood specimen