Serum diagnosis of syphilis and the butyric acid test for syphilis

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J.B. Lippincott Company, 1911 - Serum diagnosis - 238 pages
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Page 155 - ... normal sodium hydroxide. It is necessary to take the precaution to employ for this test only cerebrospinal fluid entirely free from blood. The presence of an increased content of protein in the cerebrospinal fluid is indicated by the appearance of a granular or floccular precipitate, which gradually settles to the bottom of the tube, beneath a clear, supernatant fluid. The velocity and intensity of the reaction vary according to the quantity of the protein contained in a given specimen. The greater...
Page 66 - ... blood. If there is a faint degree of hemolysis, the main bulk of corpuscles being intact, the reaction should be called positive. A more intense hemolysis, with about 10 to 20 per cent. dissolution of the corpuscle mass, should be called weakly positive, while 30 to 40 per cent, hemoh'sis is designated as faintly positive.
Page 77 - Noguchi* advocates the use of the alcohol and ether soluble but acetone insoluble extract of org.ans. This fraction contains lecithin and other phosphatids. Method of Noguchi. Extract a mashed paste of liver, heart, or kidney of man, ox, guinea-pig, rabbit, or dog with 10 parts of absolute alcohol at 37° C. for several days. Filter through filter-paper and collect the filtrate. The latter is then brought to dryness by evaporation with the aid of an electric fan or in a vacuum at 40° C. Take up...
Page 148 - ... cases still contain more than 1 antibody unit, giving strong positive reactions. However, a titration of the serum of these cases showed that they were decidedly reduced in antibody content, although they gave strong reactions. In 40 per cent of the primary cases, in 37 per cent of secondary, in 35 per cent of tertiary, in 33 per cent of latent, in 14 per cent of hereditary, and in 50 per cent of incipient tabes the reaction became negative. The average of the negative reactions equaled 33.7...
Page 207 - Ueber die Beziehungen der Syphilis zu Nerven- und anderen inneren Erkrankungen auf Grund von 573 serologischen Untersuchungen, Löwenberg, M.
Page 190 - On the Wassermann Reaction, and especially its Significance in Relation to General Paralysis.
Page 154 - Noguchi's1 butyric acid test for increase of globulin-content in the cerebrospinal fluid is as follows : Two parts of the cerebrospinal fluid to be examined are mixed with five parts of a 10 per cent. butyric acid solution in physiologic salt solution, and are heated over a flame and boiled for a brief period.
Page 155 - ... may be required to obtain a distinct reaction in specimens weaker in protein. In obtaining the reaction, the time period should not be greater than two hours.
Page 213 - The relation of protein, lipoids, and salts to the Wassermann reaction. Jour. Exper. Med., 1909, xi, 84. A new and simple method for the serum diagnosis of syphilis.
Page 193 - R. et Menard, PJ Valeur de la methode de Hecht pour le serodiagnostic de la Syphilis; comparaison avec la räaction de Wassermann.

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