Regulation of Phytochemicals by Molecular Techniques
The papers assembled in this volume were originally presented at the joint meeting of the Phytochemical Society of North America and the Mid-Atlantic Plant Molecular Biology Society, in August 2000. The symposium from which these chapters were prepared was entitled "Regulation of Phytochemicals by Molecular Techniques" and was organised by James Saunders and Ben Matthews. This joint meeting was timely because of recent landmark advances in molecular biology and genomics as well as the renewed interest in phytochemistry as a rich source of nutraceuticals, drugs, and alternatives to synthetic agriculture pesticides. Progress in genome sequencing in plants such as Arabidopsis and rice has been remarkable, as have expressed sequence tag (EST) projects in other plants, including maize and soybean. Recently, private and public sector participants of the Human Genome Project announced that a rough draft of the human genome has been constructed. These advances directly influence phytochemical investigations by providing both insight and tools for exploring and manipulating genomes.
The chapters cover a wide range of applications from molecular biology to phytochemistry, and from basic studies on promoters and gene expression to pathway regulation and engineering with transformed plants. A number of noteworthy aspects emerge from this volume: applications of molecular biology to phytochemical practical problems are succeeding; newly emerging molecular tools promise to open new doors to discovery; and remarkable progress has already occurred in phytochemical pathway engineering.
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Chapter 4 Expression of Foreign Sequences in VirusInfected Plants Using Alfalfa Mosaic Virus Coat Protein
Novel Applications for Ancient Tools
Chapter 6 Designer BasicLeucineZipper Proteins as Regulators of Plant Detoxification Genes
Chapter 7 Properties and Metabolic Engineering of Alfalfa Phenylpropanoid Pathway OMethyltransferases
Chapter 8 Mechanisms and Applications of Transcriptional Control of Phenylpropanoid Metabolism
Chapter 9 Engineering Isoprenoid Metabolism and Biochemistry in Plants
Chapter 10 Production of Cellulases in Plants for Biomass Conversion
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35S promoter Acad accumulation activity alkaloid allelochemical allelopathic AlMV CP amino acid analysis anthocyanin antibodies Arabidopsis binding Biochem Biol biosynthesis biosynthetic genes biosynthetic pathway bZIP bZIP factor CaMV carotenoid catalyzed Catharanthus roseus cDNA cellulases chalcone cloned compounds crop cyclase cytoplasmic diphosphate domain elicitor enzymes ﬁrst ﬂavonoid ﬂowers function gene expression genes encoding genetic genome glucoside glutathione identiﬁed induced infection IOMT isoﬂavone isoﬂavonoid isoprenoid levels lignin maize metabolic engineering metabolites microarray modiﬁcation molecular mosaic virus mRNA mutant Natl ORCA3 overexpression pathogens peptide phenotype phenylpropanoid phytoalexin Phytochemical phytoene Plant Cell Plant Mol Plant Physiol plastid Proc production puriﬁcation recombinant protein regulatory genes resistance resveratrol resveratrol glucoside secondary metabolism seed sequence signiﬁcant soybean species speciﬁc stilbene stilbene synthase substrate synthesis T-DNA tagging target TGAla thaliana tissue transcription factors transcriptional regulators transferase transgenic plants transgenic tobacco vaccine vacuolar vacuole vector xenobiotic yeast