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acetic acid acetin added adulteration alkali alkaloids ammonia amount amylic alcohol apparatus barium boiling boric acid butter butter-fat butyric acid c.c. of water c.c. water calculated carbonate cent Chem chloride chloroform chromic acid colour containing copper determined dextrose diluted urine required dioxide dissolved distilled Estimation ether ethyl evaporated experiments extracted fatty acids fermentation filtrate required flask glycerin gram grammes heated Hehner hydrochloric acid hydrogen invert-sugar iodine lactocrite levulose liquid method milk minutes mixed mixture neutralised nitric acid nitrogen nitrogen in 100 normal KOH obtained oxalic acid oxide oxidised paper peptone permanganate phosphate pipette platinum potash potassium precipitate present Public Analysts pure quantity reaction residue rosin salt samples saponification separation shaken Society of Public soda sodium soluble specific gravity spirit Stokes sugar sulphate sulphuric acid temperature titration toluidine tube undiluted urine Vieth vinegar volatile acids washed water-bath weighed