Recombinant DNA Research and Viruses: Cloning and Expression of Viral Genes

Front Cover
Yechiel Becker, Julia Hadar
Springer Science & Business Media, 1985 - Medical - 373 pages
1 Review
The development of recombinant DNA technology has made a marked impact on molecular virology. The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacterial p1asmids has led to the amplification of selected viral DNA fragments for sequencing and gene expression. RNA virus genomes which can be transcribed to their cDNA form were also cloned in bacterial p1asmids, facilitating the study of RNA virus genes. With the elucidation in recent years of the promoter sequence of various viral genes and the expression of these genes in bacteria or yeast, the understanding of many viral gene functions has made great progress. Cloning and expression of viral genes in mammalian cells was made possible by the construction of shuttle plasmid vectors which carry the origins of DNA replication from bacteria and/or mammalian viruses. The expression of viral genes in bacteria, yeast and eukaryotic cells gives reason to hope that it will be possible to produce viral antigens in large quantities for use as human or animal vaccines. The present volume attempts to capture for the reader some of the high lights of recombinant DNA research in the field of animal and plant viruses.
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

Cloning of Retrovirus DNA in Bacteria and Cloning of Other DNA in Retroviruses
3
Cloning of Human Oncogenes
15
Genetic Engineering Strategy
37
Development of Plasmids and Cloning Procedures
39
Cloned DNA as a Substrate of Bacterial Recombination System
59
Utilization of λ Control Elements for Gene Expression Studies in Escherichia Coli
73
Cloning of DNA Virus Genes
87
Cloning and Mapping of African Swine Fever Virus DNA
89
Cloning of Endogenous RetrovirusLike Genes The Murime VL30 Family
205
Cloning of the DNA form of an RNA Virus Genome
221
Poliovirus cDNA Cloned in Bacterial Plasmids
223
Cloning of Plant Viruses and Viroid Genes
245
Cloning and Manipulating Cauliflower Mosaic Virus
247
Cloning of Plant virus Genomes Other Than That of Cauliflower Mosaic Virus
277
Cloning of Viroid cDNA
293
Expression of Viral Genes in Bacteria
305

Cloning of the DNA of Alphaherpesvirinae
103
The Cloning and Sequencing of Sites of Linkage Between Adenovirus DNA and Cellular DNA Recombination of Foreign DNA with the Mammalian ...
125
Cloning of Papillomavirus DNA
173
Cloning of Retrovirus DNA
199
Cloning of Bovine Leukemia Virus Proviral Information
201
Expression of Cloned Genes Under Phage λ Control
307
Expression of Herpes Simplex Virus Type 1 and Type 2 Glycoprotein D Genes Using the Escherichia Coli lac Promoter
327
Cloning and Expression of Foot and Mouth Disease Virus Genes
353
Copyright

Other editions - View all

Common terms and phrases

Bibliographic information