Lipid Analysis: Isolation, Separation, Identification, and Structural Analysis of LipidsPaperback. This book is a practical guide in one compact volume to the complexities of the analysis of lipids from animal and plant tissues. Methods for chromatography, chemical analysis and spectroscopy of lipids are critically described with 900 references. The basic principles of each technique are discussed and selected procedures are described in such detail that the book should serve as a primary source, of value at the laboratory bench. Chapters follow a logical sequence from the extraction of lipids from tissues to the isolation and characteriaation of particular lipid classes and of the molecular species of each. In this second edition there is a chapter on the separation of lipoprotein classes. |
Contents
The Structure Chemistry and Occurrence of Lipids | 1 |
The Isolation of Lipids from Tissues | 15 |
Some practical considerations | 21 |
Copyright | |
15 other sections not shown
Other editions - View all
Common terms and phrases
adsorbent adsorption aldehydes alkyl amounts analysis aqueous carbon atoms cent ceramide chain chain-length Chapter Chem chemical chloride chloroform chloroform-methanol cholesterol esters Chromat column chromatography column volumes complex lipids components compounds containing derivatives described detected detector determined diacylglycerol acetates diethyl ether double bonds EGSS-X eluted enzyme esterified example extracted fatty acid composition fractions free fatty acids functional groups gangliosides gas chromatography gel G glycerides glycerol glycolipids hexane hexane-diethyl ether HPLC hydrogen hydrolysis isolated isomers lipid classes lipids liquid phases long-chain base methanol method methyl methyl esters migrate mixture moieties molecular species molecular weight molecule monoenoic necessary obtained peaks phosphatidic acid phosphatidylcholine phosphatidylethanolamine phospholipids plasmalogens polar polyunsaturated fatty acids position prepared procedure radioactivity reaction reagent removed reverse-phase saturated scintillation separated silica gel silicic acid silver nitrate simple lipids sodium solution solvent solvent system spray stationary phase technique temperature thin-layer TLC plate transesterified triacylglycerols washed