The Chromatography of Steroids
The basic theory of chromatography and some general principles; Chromatographic separation of steroids; Techniques and apparatus; Quantitative chromatography: colorimetric and radioisotopic techniques; Structural analysis and identification of steroids by chromatography; Some typical analytical problems of steroid biochemistry; Purification of reagents and materials; Microchemical reactions for steroids; Methods of detection on paper chromatograms; Calculations with Rf and Rm value.
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THE BASIC THEORY OF CHROMATOGRAPHY AND SOME GENERAL
Chemical structure and chromatographic behaviour
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17-oxosteroids acetic acid acetylation adsorbent adsorption alcohols aldosterone alkaline aqueous methanol systems ARM9 ARMg values ARMr benzene Biochem biol Bush and Mahesh Bush and Willoughby Celite cellulose Chem chloride chromato chromatogram chromic acid colour column compounds concentration conjugates cortisol derivatives elution epimeric equilibration esters estimation ether ethyl acetate evaporation extract filter paper fluorescence formamide fraction give glucuronosides hydrocarbon hydroxyl groups impregnation interactions ketone ketone groups large number less polar Lewbart Martin material methanol method methylene mixture mobile phase modifying step molecules NaOH Neher non-polar obtained oestrogens oxidation paper chromatograms Paper imp polar solvent probably procedure progesterone propylene glycol quantitative reagent Reichstein Reineke reversed-phase RF values scanning separation silica gel sodium solubility solution solvent systems specific stationary phase steric steroids strip substance substituents sulphate t-butanol Tait technique tetrahydrocortisol toluene typical unpublished urine usually volatile washed Zaffaroni zone