Basic BiotechnologyColin Ratledge, Bjorn Kristiansen Biotechnology is one of the major technologies of the twenty-first century. Its wide-ranging, multi-disciplinary activities include recombinant DNA techniques, cloning and the application of microbiology to the production of goods from bread to antibiotics. In this new edition of the textbook Basic Biotechnology, biology and bioprocessing topics are uniquely combined to provide a complete overview of biotechnology. The fundamental principles that underpin all biotechnology are explained and a full range of examples are discussed to show how these principles are applied; from starting substrate to final product. A distinctive feature of this text are the discussions of the public perception of biotechnology and the business of biotechnology, which set the science in a broader context. This comprehensive textbook is essential reading for all students of biotechnology and applied microbiology, and for researchers in biotechnology industries. |
Contents
| 25 | |
Stoichiometry and kinetics of microbial | 55 |
yeasts | 119 |
Microbial process kinetics | 155 |
Bioreactor design | 181 |
Mass transfer | 201 |
Downstream processing | 219 |
Measurement monitoring modelling | 251 |
Microbial polysaccharides and single | 381 |
Environmental applications | 403 |
Production of antibiotics by fermentation | 433 |
Strategies of cultivation | 457 |
Enzyme biotechnology | 477 |
Recombinant proteins of high value | 499 |
Insect and mammalian cell culture | 523 |
Plant cell biotechnology | 549 |
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Common terms and phrases
acetyl-CoA activity affinity amino acids anaerobic antibiotics antibody antigen applications bacteria biocatalyst biomass biomass concentration bioreactor biotechnology cell culture cell-line cephalosporins cerevisiae chemical chemostat chromosome citric acid cloning coefficient coli complex compounds cost defined dehydrogenase difficult efficient encoding energy engineering enzyme example expression fatty acids fed-batch feed fermentation Figure filamentous filter final first flow fluid flux formation function fungal fungi gene genetic genome Gibbs energy gluconic acid glucose immobilised increase industrial influence insect l-lysine liquid mammalian mass transfer medium membrane metabolism metabolites microbial microorganisms modified molecules mutant nutrients optimisation organic oxygen parameters pathway penicillin phage phase plant cell plasmid polysaccharides production protein purification pyruvate reaction reactor recombinant sequence significant sludge solvents species specific specific growth rate stirred tank strains substrate sufficient synthesis target techniques temperature tion transcription vector yeast yield