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The components of the NPC and their role in nucleocytoplasmic transport
FG Nucleoporins as the docking sites during the transport process
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affinity resin affinity-capture assay Allen antibodies bait binds directly Biol bound proteins buffer cargo cell cycle cell cycle conditions cell cycle dependent cell extracts cerevisiae coli concentration Crm1p Cse1p cytoplasmic cytoplasmic fibrils data not shown eluate eluted exportin FG nucleoporins FG Nups FG region FG repeat regions Figure fraction fusion FxFG GLFG region gradient Gsp1 Gsp1p-GTP GST-fusions GTPase system heterodimers histones homolog identified importin incubated interacting proteins Kaps karyopherins KOAc lane lndeed mass spectrometry molecules mRNA Msn5p mutant NaCl Nmd5p nuclear basket structure nuclear envelope nuclear export nuclear pore complex nucleocytoplasmic transport nucleoplasm nucleoporins nucleus Nup1p Nup2p Nup84p complex p-GTP Pab1p Pablp peptide repeats permeability barrier protein interactions proteins bound proteins captured proteomic PSFG purified proteins Ran-GTP RCC1 recombinant region of Nup116p resolved by SDS-PAGE Rna1 Rna1p role SDS-PAGE specific subunit Sxm1p tethered translocation two-hybrid ug/ml vivo western blots yeast extracts Yrb1p