Networks of Protein Interactions at the Nuclear Pore Complex in Saccharomyces CerevisiaeStanford University, 2003 - 272 pages |
Contents
Introduction | 2 |
The components of the NPC and their role in nucleocytoplasmic transport | 5 |
FG Nucleoporins as the docking sites during the transport process | 8 |
Copyright | |
17 other sections not shown
Common terms and phrases
affinity resin affinity-capture assay Allen antibodies bait binds directly bound proteins buffer cargo cell cycle cell cycle conditions cell cycle stage cell extracts cerevisiae coli Crm1p Cse1p cytoplasmic cytoplasmic fibrils data not shown dependent manner Eft2p eluted exportin FG nucleoporins FG Nups FG region FG repeat regions Figure fraction fusion FxFG gradient Gsp1p Gsp1p-GTP GST-fusions heterodimers histones homolog identified importin incubated interacting proteins Kaps karyopherins KOAc lane mass spectrometry mRNA Msn5p mutant Nab4p NaCl eluate networks of protein Nmd5p Nsp1p nuclear basket structure nuclear envelope nuclear import nuclear pore complex nucleocytoplasmic transport nucleoplasmic nucleoporins nucleus Nup1p Nup2p Nup84p complex peptide repeats permeability barrier protein interactions proteins captured proteomic Prp20p and Gsp1p PSFG purified proteins RCC1 recombinant region of Nup116p resolved by SDS-PAGE Rna1p role SDS eluate SDS-PAGE Seh1p soluble specific Ssa2p subunit Sxm1p tethered translocation two-hybrid vivo western blots yeast extracts Yrb1p µg/ml