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analysis antigen binding Biol biological bottom phase buffer buoyant density cell populations cell separation cell surface cell volume cellular centrifugation charge chloroplasts concentration conductivity Countercurrent distribution Crissman cytoplasmic cytoplasmic diameter DCR spectrum density gradient dextran dielectric dielectrophoresis DNA content effects electric field electrode electrostatic potential difference erythrocytes experiments fibers Ficoll fluorescence fractions function g/ml H. A. Pohl interfacial isokinetic gradient isopycnic lectin leukocytes ligand light scatter light-scatter living cells lymphocytes lysosomes MCS type measured membrane method mitochondria mobility mouse spleen NaCl nonuniform electric field normal number of cells observed obtained ohm-m organelles parameters particles partition coefficient peak Pertoft phase system Phys platelets polarization polymer Pretlow protein purification ratio receptors red blood cells red cells region Rutishauser salt separation of cells Shortman shown in Fig solution specific spleen Steinkamp studies sucrose suspension technique tion top phase tube tumor velocity sedimentation voltage Walter yeast cells