Animal Cell Culture: A Practical ApproachR. Ian Freshney |
From inside the book
Results 1-3 of 62
Page 19
... acid 16 E - 9 , L - cell supernatant 30 % Purified human serum albumin 2.5-10 mg / ml 2 - Mercaptoethanol 1.0 E - 5 , 2 - aminoethanol 1.0 E - 5 , human low density lipoprotein 1-2 μg / ml , oleic acid 4 μg / ml complexed to fatty acid ...
... acid 16 E - 9 , L - cell supernatant 30 % Purified human serum albumin 2.5-10 mg / ml 2 - Mercaptoethanol 1.0 E - 5 , 2 - aminoethanol 1.0 E - 5 , human low density lipoprotein 1-2 μg / ml , oleic acid 4 μg / ml complexed to fatty acid ...
Page 40
... acid , but it usually results in a slower growth rate . These precautions delay the onset of a non - physiological pH and are sufficient for small cultures . As scale- up increases , headspace volume and culture surface area in relation ...
... acid , but it usually results in a slower growth rate . These precautions delay the onset of a non - physiological pH and are sufficient for small cultures . As scale- up increases , headspace volume and culture surface area in relation ...
Page 218
... acid site since it is essentially a probe excess method , so that even minor impurities may hybridise . Whereas standard physical methods can produce probes of high purity from abundant nucleic acids such as ribosomal RNA , the ...
... acid site since it is essentially a probe excess method , so that even minor impurities may hybridise . Whereas standard physical methods can produce probes of high purity from abundant nucleic acids such as ribosomal RNA , the ...
Contents
Preparation | 7 |
TOWARDS CHEMICALLYDEFINED SERUMFREE MEDIA | 13 |
Practical Suggestions for Medium Selection and Handling | 26 |
Copyright | |
14 other sections not shown
Other editions - View all
Common terms and phrases
agar ampoules assay autoradiographic buffer cell culture cell cycle cell lines cell number cell populations cell suspension cell type centrifugation chamber cloning clonogenic clonogenic assays clot cm² concentration containing coverslip cytotoxicity detection diameter differentiation diluted disaggregation distilled water drug elutriation embryos emulsion enzyme epithelium ethanol explants exposure Figure filter flow cytometry flow rate fluorescence forceps fraction glass growth factors growth medium HBSS hormones human tumour hybridisation hybridoma incubation laboratory laser lens paper measure method microcarrier mixing monolayer obtained organ culture oxygen Pasteur pipettes perfusion Petri dishes pipette plasma plate prepared probe procedure proliferation propidium iodide prostate protein pump Reagents remove rinse rotor sample sensitivity serum slides solution specific spheroids stain sterile stromal cells surface technique Traganos transfer trypsin tubes tumour cell viability vitro vivo volume wash watchglass µg/ml