Animal Cell Culture: A Practical ApproachR. Ian Freshney |
Contents
Preparation | 7 |
TOWARDS CHEMICALLYDEFINED SERUMFREE MEDIA | 13 |
Practical Suggestions for Medium Selection and Handling | 26 |
Copyright | |
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agar ampoules assay autoradiographic buffer cell culture cell cycle cell lines cell number cell populations cell suspension cell type centrifugation chamber cloning clonogenic clonogenic assays clot cm² concentration containing coverslip cytotoxicity detection diameter differentiation diluted disaggregation distilled water drug elutriation embryos emulsion enzyme epithelium ethanol explants exposure Figure filter flow cytometry flow rate fluorescence forceps fraction glass growth factors growth medium HBSS hormones human tumour hybridisation hybridoma incubation laboratory laser lens paper measure method microcarrier mixing monolayer obtained organ culture oxygen Pasteur pipettes perfusion Petri dishes pipette plasma plate prepared probe procedure proliferation propidium iodide prostate protein pump Reagents remove rinse rotor sample sensitivity serum slides solution specific spheroids stain sterile stromal cells surface technique Traganos transfer trypsin tubes tumour cell viability vitro vivo volume wash watchglass µg/ml