Animal Cell Culture: A Practical ApproachR. Ian Freshney |
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Page 118
... fractions as they are collected and indicates , when free of cells , that a subsequent fraction can be elutriated . This also ensures that discrete fractions are collected in a minimum volume . 3.4 Complete Elutriation System The ...
... fractions as they are collected and indicates , when free of cells , that a subsequent fraction can be elutriated . This also ensures that discrete fractions are collected in a minimum volume . 3.4 Complete Elutriation System The ...
Page 121
... fraction . About 150 ml of elutriation buffer may be needed to collect all of the cell fraction . When the cell boundary is again visible in the separation chamber and the flow cell is free of cells , a further incremental increase in ...
... fraction . About 150 ml of elutriation buffer may be needed to collect all of the cell fraction . When the cell boundary is again visible in the separation chamber and the flow cell is free of cells , a further incremental increase in ...
Page 122
... fractions elutriated at the flow rates shown . 5.2 Established Cell Lines ( Recovery and Viability ) Alternative ... fraction of G1 cells . Both G1 cells and early 600 Gr CELLS 600- G1 CELLS INCUBATED 2h @ 36 122 Separation of Viable ...
... fractions elutriated at the flow rates shown . 5.2 Established Cell Lines ( Recovery and Viability ) Alternative ... fraction of G1 cells . Both G1 cells and early 600 Gr CELLS 600- G1 CELLS INCUBATED 2h @ 36 122 Separation of Viable ...
Contents
Preparation | 7 |
TOWARDS CHEMICALLYDEFINED SERUMFREE MEDIA | 13 |
Practical Suggestions for Medium Selection and Handling | 26 |
Copyright | |
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agar ampoules assay autoradiographic buffer cell culture cell cycle cell lines cell number cell populations cell suspension cell type centrifugation chamber cloning clonogenic clonogenic assays clot cm² concentration containing coverslip cytotoxicity detection diameter differentiation diluted disaggregation distilled water drug elutriation embryos emulsion enzyme epithelium ethanol explants exposure Figure filter flow cytometry flow rate fluorescence forceps fraction glass growth factors growth medium HBSS hormones human tumour hybridisation hybridoma incubation laboratory laser lens paper measure method microcarrier mixing monolayer obtained organ culture oxygen Pasteur pipettes perfusion Petri dishes pipette plasma plate prepared probe procedure proliferation propidium iodide prostate protein pump Reagents remove rinse rotor sample sensitivity serum slides solution specific spheroids stain sterile stromal cells surface technique Traganos transfer trypsin tubes tumour cell viability vitro vivo volume wash watchglass µg/ml