Animal Cell Culture: A Practical ApproachR. Ian Freshney |
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... Technique 151 Preparation of embryo extract 151 Preparation of chicken plasma 152 Explantation The Maximow Single Slide Technique Materials Setting up of cultures 153 156 156 157 Renewal of medium Agar Gel Techniques Embryological ...
... Technique 151 Preparation of embryo extract 151 Preparation of chicken plasma 152 Explantation The Maximow Single Slide Technique Materials Setting up of cultures 153 156 156 157 Renewal of medium Agar Gel Techniques Embryological ...
Page 151
... technique devised by Trowell ( 17 ) . He introduced metal grids , made at first of tantalum wire gauze . This was replaced later by the more rigid expanded metal , obtainable as a continuous sheet of stainless steel ( 18 ) , or by ...
... technique devised by Trowell ( 17 ) . He introduced metal grids , made at first of tantalum wire gauze . This was replaced later by the more rigid expanded metal , obtainable as a continuous sheet of stainless steel ( 18 ) , or by ...
Page 247
... technique , grid techniques 161 Trypan blue , Trypsin , membrane integrity 193 enzymic disaggregation of solid tissues 138 use in primary culture preparation 9 Tubing , scale - up equipment 35 Tumorigenicity , continuous cell line 5 ...
... technique , grid techniques 161 Trypan blue , Trypsin , membrane integrity 193 enzymic disaggregation of solid tissues 138 use in primary culture preparation 9 Tubing , scale - up equipment 35 Tumorigenicity , continuous cell line 5 ...
Contents
Preparation | 7 |
TOWARDS CHEMICALLYDEFINED SERUMFREE MEDIA | 13 |
Practical Suggestions for Medium Selection and Handling | 26 |
Copyright | |
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agar ampoules assay autoradiographic buffer cell culture cell cycle cell lines cell number cell populations cell suspension cell type centrifugation chamber cloning clonogenic clonogenic assays clot cm² concentration containing coverslip cytotoxicity detection diameter differentiation diluted disaggregation distilled water drug elutriation embryos emulsion enzyme epithelium ethanol explants exposure Figure filter flow cytometry flow rate fluorescence forceps fraction glass growth factors growth medium HBSS hormones human tumour hybridisation hybridoma incubation laboratory laser lens paper measure method microcarrier mixing monolayer obtained organ culture oxygen Pasteur pipettes perfusion Petri dishes pipette plasma plate prepared probe procedure proliferation propidium iodide prostate protein pump Reagents remove rinse rotor sample sensitivity serum slides solution specific spheroids stain sterile stromal cells surface technique Traganos transfer trypsin tubes tumour cell viability vitro vivo volume wash watchglass µg/ml