Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular BiologyFrederick M. Ausubel |
Contents
ESCHERICHIA COLI | 1-3 |
II | 1-13 |
THE POLYMERASE CHAIN REACTION | 1-15 |
Copyright | |
52 other sections not shown
Common terms and phrases
acid acrylamide agar agarose gel aliquots ALTERNATE PROTOCOL Analysis of DNA antibody antigen assay bacterial Bacteriophages basic protocol buffered phenol cDNA centrifuge cloning column concentration containing Contributor culture deletions denaturing digestion dilution DNA fragments DNA ligase DNA polymerase DNA Sequencing dNTPs EcoRI EDTA elution Enzymatic Manipulation Escherichia coli ethanol precipitate ethidium bromide extract filter gel electrophoresis gene gradient hybridization ice-cold incubate insert Klenow fragment labeled ligation Manipulation of DNA Materials see APPENDIX medium mg/ml microcentrifuge tube NaCl nitrocellulose nuclease nucleotides oligonucleotide overnight phage phenol pipet plaques plasmid plasmid DNA Preparation and Analysis primer probe protein purified radioactive reagents Remove restriction endonuclease Rinse RNase room temperature rotor sample single-stranded sodium acetate solution step strain supernatant SUPPORT PROTOCOL synthesis T7 DNA polymerase template titer transfection Transfer Tris-Cl UNIT vector vector DNA viral virus volume vortex wash µg/ml