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absorption acid activity added analyte antibodies antigen application assay binding buffer BzAF calculated cells Chem complex concentration conjugate constant containing counting curve decay dependence described detection detector determined developed device diode distribution effect efficiency emission energy enzyme excitation experiments fiber fiber optic Figure filter fluorescence fluorescence intensity fluorescence lifetime fluorophore frequency function immobilized immunoassay increase indicator intensity interaction labeled laser lifetime light limit luminescence measurement method modulation molecular molecules monitored myosin observed obtained optical output oxygen peak performed phase phosphorescence photon polarization possible prepared present probe protein pulse quenching range ratio reaction reference reflectance relative respectively response sample scattering selectivity sensing sensitivity sensor shift shown shows signal single solution specific spectra spectrum standard structure studies surface Table technique temperature transfer wavelength