Methods in Gene Technology, Volume 1
J.W. Dale, P.G. Sanders
Elsevier Science, Nov 3, 1991 - Science - 307 pages
A series which reviews the most recent methods and applications available for generating probes, as well as established procedures. Volume 1 discusses hybridization, the generation of labelled probes, and the uses and methods of detection of isotopic and non-isotopic probes.
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added addition alkaline allow amount amplification analysis appropriate approximately background bacteriophage bands base biotinylated blotting buffer carried cDNA cells Chapter clones complete concentration containing cycles denatured depends described detection digestion DNA probes EDTA efficiency electrophoresis enzyme ethanol experiments Figure filter final formamide fragment full-length gene genome give heating human hybridization incorporation increase incubation labelled length mapping membrane method molecular molecules mRNA necessary Note nuclease Nucleic Acids Res nucleotide oligonucleotide PACA placed plate polymerase possible prepared present primer problem procedure protection protocol radioactive reaction reaction mix region remove restriction RNase samples screen sensitivity sequence single-stranded solution specific activity standard step stored strand synthesis technique temperature template tion tissue transcription transfer Tris-HCl tube University usually vector volume wash