A Genetic Switch: Phage Lambda RevisitedThe first edition of Mark Ptashne's 1986 book describing the principles of gene regulation in phage lambda became a classic in both content and form, setting a standard of clarity and precise prose that has rarely been bettered. This edition is a reprint of the original text, together with a new chapter updating the story to 2004. Among the striking new developments are recent findings on longā€“range interactions between proteins bound to widely separated sites on the phage genome, and a detailed description of how gene activation works. |
Contents
| 4 | |
| 11 | |
CHAPTER | 31 |
CHAPTER THREE | 47 |
CHAPTER FOUR | 67 |
Repressor Isolation and DNA Binding | 72 |
A repressor dimer binds through its amino domains to a 17 base pair | 78 |
There are three 17 base pair repressor binding sites in the right operator | 84 |
Cro binds first to Or3 then to OR1 and OR2 thereby first turning off | 92 |
CHAPTER FIVE | 109 |
Index | 151 |
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Common terms and phrases
a-helix activator affinity amino acids amino domain bacterial bacterium base pairs bearing binds cooperatively Biol bound carboxyl domain cells Chapter chromosome cl gene cleavage cleaves clll coli contacts cooperative binding Cro bind described dimers bind DNA molecule DNA-binding effect encoding enzyme example experiment gene expression gene regulation genetic half-site helix immune induction infection interactions IPTG irradiation lac repressor Lambda LexA linker lyse lysogen lytic growth major groove merase methylation monomers mRNA mutant Natl nonspecific octamer operator sites OR1 and OR2 OR3 OR2 OR1 P22 repressor phage chromosome phage genes phosphate plasmid polymerase bind positive control promoter prophage protease protein-DNA Ptashne RecA recognition helix region regulatory protein repres repression repressor and Cro repressor at OR2 repressor binds repressor concentration repressor dimer repressor monomers residues RNA polymerase sequence shown in Figure shows specific strand structure switch synthesis tein tetramers tion transcribed turns vitro wild-type
Popular passages
Page 141 - Other examples of the contribution of secondary products to chemosystematics can be found in the references cited at the end of the chapter but this is an area in which there is great scope for further research.
Page 147 - RIGGS. 1975. The general affinity of lac repressor for E. coli DNA: Implications for gene regulation in prokaryotes and eukaryotes. Cell 4: 107.
Page 105 - Ingles. 1985. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases. Cell 42:599-610.
Page 106 - Meyer BJ, Maurer R., and Ptashne M. 1980. Gene regulation at the right operator (OR) of bacteriophage lambda.
Page 105 - DNase footprinting: a simple method for the detection of protein-DNA binding specificity. Nucl. Acids. Res.
Page 137 - DL (2001). Bacteriophage lambda: Alive and well and still doing its thing. Curr. Opin. Microbiol 4, 201-207. 2. Hendrix, RW, Roberts, JW, Stahl, JW, and Weisberg, RA (Eds.) (1983). Lambda II. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 3. Ptashne, M. (1992). "A Genetic Switch,
Page 29 - R. 1983. Represser and Cro protein: Structure, function, and role in lysogenization. In Lambda II, ed.
Page 105 - Maximizing gene expression on a plasmid using recombination in vitro.
Page 106 - CALEF, 1970 Immunity phase-shift in defective lysogens: non-mutational hereditary change of early regulation of X prophage.
Page 46 - Siebenlist U., Simpson RB, and Gilbert W. 1980. E. coli RNA polymerase interacts homologously with two different promoters. Cell 20: 269-281.