GB 5009.212-2016: Translated English of Chinese Standard. GB 5009.212-2016: National Food Safety Standard - Determination of Diarrheic Shellfish Poison in Shellfish
https://www.chinesestandard.net, Jun 18, 2019 - Technology & Engineering - 22 pages
This Standard specifies mouse biology method, enzyme linked immunosorbent assay (ELISA method) and liquid chromatography - tandem mass spectrometry for the determination of diarrheic shellfish poison in shellfish. In this Standard, mouse biology method and enzyme linked immunosorbent assay (ELISA method) are applicable to the determination of diarrheic shellfish poison in shellfish and shellfish products; liquid chromatography - tandem mass spectrometry is applicable to the determination of diarrheic shellfish poison okadaic acid (OA), dinophysistoxins-1 (DTX-1) and dinophysistoxins-2 (DTX-2) in edible part of shellfish and shellfish products (excluding salted products).
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Liquid Chromatography Tandem Mass Spectrometry
Instruments and Equipment
Expression of Analytical Result
Appendix A Evaluation Technical Parameters of Commercial Kit
Appendix B Virulence Factor of Diarrheic Shellfish Poison
Multiple Reaction Monitoring Chromatogram of Diarrheic Shellfish Poison Standard Solution
Other editions - View all
accordance with Formula accordance with Table accurately weigh-take acetone acetonitrile ammonium formate anhydrous ether assay ELISA method chromatographic purity concentration of diarrheic constant volume cross reaction rate Determination of Diarrheic diarrheic shellfish poison Diluent Dinophysistoxins-2 DTX-2 Disodium hydrogen phosphate enzyme linked immunosorbent enzyme marker Homogenize Hydrochloric acid immunosorbent assay ELISA linked immunosorbent assay liquid chromatography mass concentration measure-take Methanol solution 90 mL of methanol mL of water Mobile phase Mouse Biology Method ng/mL Okadaic acid OA open the shell PBS solution Poison in Shellfish poison standard solution Potassium dihydrogen phosphate quantitation limit r/min reach a constant rinse room temperature round-bottomed flask Sample Extraction sample solution series working solution shell meat shellfish poison standard SN/T Sodium hydroxide solid phase extraction solution 2.5 mol/L solvent control group standard substance standard working solution Sulfuric acid tandem mass spectrometry test group test stock solution Tween-60 saline solution various diarrheic shellfish Virulence Factor water to dilute