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Applications of the Polymerase Chain Reaction in RetroviralMediated Gene Transfer and the Analysis
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Acad amphotropic analysis assays BEAC bone marrow cancer cDNA cell gene therapy CFU-S clinical concems Congress culture deﬁnition detected developed efﬁcient embryos endothelial cells ethical experiments ﬁbroblasts ﬁrst gene expression gene transfer genetic engineering genome germ-line helper virus hematopoietic cells HSV-TK human gene therapy human gene transfer human genetic engineering infected inserted levels lymphocytes lymphoma marker modiﬁcation molecular Moolten mouse murine leukemia virus Natl neomycin NeoR gene Ofﬁce oncogene patients plasmid potential President’s Commission primer Proc produced progenitor cells protein protocol proviral provirus Recombinant DNA retroviral vectors retroviral-mediated gene transfer samples scientiﬁc selection sequences somatic cell somatic cell gene Southem blot speciﬁc spleen stem cells Subcommittee target TEMIN thymidine kinase tissues titer transcription transduced transduced cells transgenic transgenic mice transplanted treatment tumor untransduced vector containing viral Virol viruses vitro vivo