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12 days acetate ACTH content activity administration adrenal ascorbic acid adrenal corticosterone content adrenal gland adrenal weight adult age groups analysis animals ascorbic acid depletion assay birth body weight caused changes cholesterol implanted compared continue days of age decrease demonstrated detectable determination differences doses early effects electric shock elevation epinephrine estimate evidence experiment exposure fetal adrenal findings function gestation higher hormonal hydrocortisone implanted implanted females implanted mothers increase indicate injection levels Levine litter lower marked maternal mean measured method mice Milkovic mouse ND ND neonatal newborn normal observed obtained occurred offspring pattern period pituitary ACTH pituitary-adrenal system placed plasma corticosterone concentration position postnatal pregnancy present procedure pups rats relative release removed reported represent response to stress resting saline seen sensitivity showed significant significantly standard steroids TABLE tissue tube values variance