Monoclonal Antibodies: Principles and Practice

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Elsevier, Feb 26, 1996 - Medical - 492 pages
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Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.

The Third Edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an integrated treatment of both the production and application of monoclonal antibodies. As in previous editions, detailed and critical accounts of the theory, production, purification, fragmentation, storage and radiolabelling of monoclonal antibodies are given, along with descriptions of their use in antigen characterization, affinity chromatography and immunofluorescence. The present volume has been comprehensively updated to cover recent rapid advances, particularly with respect to the applications of molecular biology, the use of antibodies in closing and heterologous expression of genes, immunohistology and phage display libraries. Since the previous edition, there has been a growing trend towards the replacement of procedures using radioactive isotopes, and the current edition incorporates these newer technologies.

The text is oriented towards problems solving, and makes it easy to adapt each procedure to individual needs. Extensive cross-referencing, a glossary and a comprehensive index make this book an essential reference.

This book will be vital both for laboratories already producing or using monoclonal antibodies, and for workers in many disciplines who are contemplating their use.

  • Provides an integrated treatment of both the production and application of monoclonals in cell biology, biochemistry, and immunology
  • Gives detailed and critical accounts of the theory, production, purification, storage, and relabelling of monoclonals, and their use in antigen characterization, affinity chromorography, and immunofluroscence
  • Comprehensively updated to cover the rapid advances that have occurred since the publication of the Second Edition
 

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Contents

Chapter 1 Introduction
1
Chapter 2 The Antibody Response
7
Chapter 3 Cellular Basis of the Immune System
26
Chapter 4 Nature of Antigens
50
Chapter 5 Antibody Structure and Function
72
Chapter 6 Genetics of Antibodies
101
Chapter 7 Introduction to Monoclonal Antibodies
116
Chapter 8 Production of Monoclonal Antibodies
141
Chapter 10 Analysis of Antigens Recognized by Monoclonal Antibodies
234
Chapter 11 Affinity Chromatography
327
Chapter 12 Immunofluorescence
352
Chapter 13 Immunohistology
400
Chapter 14 Construction Screening and Expression of Recombinant Antibodies
424
Chapter 15 Generation of Conventional Antibodies
465
Glossary
480
Index
485

Chapter 9 Purification Fragmentation and Isotopic Labelling of Monoclonal Antibodies
192

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