Basic Methods in Molecular Biology1: The basics of molecular biology. 2: The tools of the molecular biologist. General preparations, procedures, and considerations for use of manual. 4: Cloning vectors and bacterial cells. Preparation of DNA form eukaryotic cells. 6: Probing nucleic acids with labeled synthetic probes. 7: Probing nucleic acids with plasmid-derived probes. 8: Plasmid DNA preparation. 9: DNA Restriction fragment preparation. 10: Purification of DNA. 11: Preparation and analysis of RNA from eukaryotic cells. 12: Preparation of DNA from bacteriophage clones. Cloning DNA from the eukaryotic genome. 14: cDNA cloning into gt10 and gt11. 15: Subcloning into plasmids. 16: M13 cloning and sequencing. 17: Further characterization of cloned DNA. 18: Transfection of mammalian cells in culture. 19: Protein methods. 20: General methods. Specialized methods. |
Contents
Cloning Vectors and Bacterial Cells | 23 |
Section 51 Rapid DNA Preparation | 42 |
Probing Nucleic Acids with Labeled Synthetic Probes | 67 |
Copyright | |
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Basic Methods in Molecular Biology Leonard G. Davis,Mark D. Dibner,James F. Battey Snippet view - 1992 |
Common terms and phrases
acetate acid added additional Advance agar agarose aliquots allow analysis apparatus appropriate approximately aqueous bacterial bacteriophage band bases blot buffer cDNA cells centrifugation chloroform cloning coli colonies column concentration containing culture described DESCRIPTION determine digestion dilution EcoRI EDTA electrophoresis EQUIPMENT ethanol ethidium extraction filter fragments gene genomic glass Grow growth H₂O host hybridization Incubate insert interest ligation loading medium method mg/ml microcentrifuge microfuge NaCl needed Note overnight packaging pellet phage phase pipette Place plaques plasmid plastic plate polymerase positive precipitate Prepare probe procedure protein purified reaction REAGENTS REFERENCES region Remove REQUIRED Resuspend room temperature sample screening Section selection separate sequence sodium solution specific SS-phenol step sterile Store supernatant tion tissue Transfer transformation Tris tube upper vacuum vector volume wash
References to this book
PCR : a practical approach. 1 M. J. McPherson,Graham R. Taylor,Philip Quirke No preview available - 1991 |