Promoter Sequences that Affect the Access and Maintain the Binding of Heat Shock Factor to Heat Shock Elements in DrosophilaCornell University, 1996 - 262 pages |
Contents
Heat shock elements and the heat shock promoter | 20 |
Indirect immunofluorescence | 32 |
footprinting in nuclei | 37 |
Copyright | |
1 other sections not shown
Common terms and phrases
accessible activation additional affect affinity amounts analysis anti-dHSF anti-pol antibody appear assays association attenuates bound Cell Chapter chromatin structure chromosomes complex conserved contains Continued coordinated core promoter deleted dependent described detected determine digested direct dissociates distribution dmHSE-L1 DNAse domain Drosophila endogenous et al examined expression factor Figure fluorescence signal footprinting GAGA elements heat shock gene heat shock promoters HSE polymer HSF binding hsp70 promoter HSPs identified indicate indirect immunofluorescence influence inserted interactions J. T. Lis kinetics lane levels lines located loci locus maintain measurements mmGAGA-L1 mutations nuclei observed pattern paused polymerase portion presented previously protein puffs region regulated relative repeats reported response result RNA polymerase RNA polymerase II role run-on selected sequences shock gene transcription shown similar specific staining suggest Table TATA TFIID transcribed transcription factor transgenic trimers units upstream vitro vivo Xiao