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Glucosesensing proteins from mesophilic and thermophilic bacteria as new tools in diabetes
Surfaceenhanced fluorescence biochips using industrial standard slide format and scanners
Duallaser fluorescence correlation spectroscopy as a biophysical probe of binding
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absorption addition analysis analyte anisotropy antibody applications approach approximately assay autofluorescence binding bioluminescence bound buffer cell channel chemical cluster complex compound concentration containing decay decrease dependence described detection detector determined developed distance distribution function elements enhancement excitation excitation wavelength experimental Figure filter flow fluorescence emission fluorescence intensity fluorescence lifetime fluorescence measurement fluorescence signal fluorescent probe fluorophores formation FRET function glass glucose immobilized increase indicates intensity interaction interference labeled Lakowicz laser layer levels ligand light macrophages measurement metal method microspheres modulation molecules monitoring observed obtained optical particle performed phagocytized microspheres phase polarization possible present protein pulsed range reaction red fluorescence reference reported respectively response sample selected sensing sensitive sensor shown shows signal silica single solution specific spectra spectroscopy spectrum standard structure surface technique Technology Thompson time-resolved tissue wavelength yield