T Cell Protocols: Development and Activation
Kelly P. Kearse
Springer Science & Business Media, 2000 - Medical - 365 pages
In T Cell Protocols: Development and Activation, Kelly P. Kearse and a panel of laboratory experts describe the step-by-step execution of a wide variety of basic and specialized techniques for the study of T cell developmental biology and of their subsequent activation. Readily reproducible, the techniques are explained concisely, but in such detail that they are suitable for both the novice and experienced investigator. Coverage includes treatment of both fundamental and novel experimental protocols for the analysis of the role T cells play in immune function and in the prevention and cure of human diseases. Practical and timely, T Cell Protocols: Development and Activation offers basic scientists and clinical investigators a valuable compendium of key protocols that will improve skills with existing methods, as well as afford rapid mastery of powerful new techniques.
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analysis Annexin anti-CD3 antibody antigen antigen receptor apoptosis apoptotic assay beads Becton Dickinson Biology CD8+ cDNA cell lines Cell Protocols cell receptor cell surface cell suspension centrifuge clones column complex conjugated containing cuvet cytokines cytoplasmic dendritic cells detection Development and Activation differentiation dilution electroporation EtBr expression fetal filter flow cytometry fluorescence gene Gibco-BRL human hybridization hybridomas immature immune Immunol Immunology immunoprecipitated Incubate integrin intracellular isolated K. P. Kearse labeled ligands lineage lymphocytes lymphoma lysis buffer measurements medium membrane MHC class mice molecules mouse murine negative selection Note PBMC peptide phenotype phosphatase phosphorylation pipet plasmid plate population precursor probe procedure protein reagents remove Resuspend room temperature sample signaling solution specific staining sterile stimulation Subheading subsets supernatant T-cell T-cell development thymic thymocytes thymus thymus lobes tissue culture transfection transgenic tube tyrosine kinases ubiquitination vector washing buffer