Live Cell Imaging: A Laboratory Manual

Robert D. Goldman, Jason Swedlow, David L. Spector

Cold Spring Harbor Laboratory Press, 2010 - Science - 736 pages

The second edition of Live Cell Imaging: A Laboratory Manual expands upon and extends the collection of established and evolving methods for studying dynamic changes in living cells and organisms presented in the well-known first edition. There are 16 new chapters and the 21 updated chapters in this new edition. They include advances in atomic force microscopy, structured illumination microscopy and other 3-D approaches, as well as imaging in single cells in animals and in plants. New analytical options include live high-throughput/high-content screening in mammalian cells and computational analysis of live cell data. The manual presents hands-on techniques as well as background material, and can serve as a text in advanced courses. The first section covers principles and fundamental issues of detection and imaging; the second provides detailed protocols for imaging live systems.

About the author

Robert D. Goldman is an American cell and molecular biologist. He is the Chair of the Department of Cell and Molecular Biology at Northwestern University Feinberg School of Medicine and holds the Stephen Walter Ranson Professor of Cell Biology at the institution.

Bibliographic information

• Title: Live Cell Imaging: A Laboratory Manual; Cold Spring Harbor Laboratory Series; G - Reference,Information and Interdisciplinary Subjects Series
• Editors: Robert D. Goldman, Jason Swedlow, David L. Spector
• Edition: 2, illustrated
• Publisher: Cold Spring Harbor Laboratory Press, 2010
• ISBN: 0879698934, 9780879698935
• Length: 736 pages
• Subjects: Science / Life Sciences / Cell Biology; Science / Microscopes & Microscopy; Science / Research & Methodology