Techniques in Glycobiology

Front Cover
R. Reid Townsend, Arland T. Hotchkiss
CRC Press, Jun 25, 1997 - Science - 648 pages
This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.
 

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Contents

Mass Spectrometry and
1
Analysis of Anionic Glycoconjugates by Delayed Extraction
17
MatrixAssisted Laser Desorptionlonization TimeofFlight
33
The Fragmentation Behavior of Glycopeptides Using
53
Application of Electrospray lonization Mass Spectrometry
67
Structural
85
Glycolipids and New Physical Methods
111
HighPerformance Liquid Chromatography of Gangliosides
127
Analysis of Permethylated Glycoprotein Oligosaccharide
335
Highly Sensitive PreColumn Derivatization Procedures
349
Glycan Labeling with the Fluorophores 2Aminobenzamide
359
Capillary Gel Electrophoresis of 8Aminopyrene368
377
Evaluation of Glycosylpyrazole Derivatives
391
Strategies for Oligosaccharide Separations and Sequencing
401
Resolution of Oligosaccharides in Capillary Electrophoresis
409
A Comparison of Oligosaccharide Profiling Methods
431

A Sensitive Cell Suspension EnzymeLinked Immunosorbent
137
Scattering Techniques for the Study of Aggregative Properties
167
Imaging Carbohydrate Polymers with Noncontact Mode
187
Novel Approach for the Analysis of Interaction Between
209
Glycoprotein Detection Using the LightAddressable
229
Protein 0Glycosylation
247
Predicting MucinType OGlycosylation Sites
271
Experimental Approaches to Studying OGlycosylation In Vivo
289
Identifying Sites of Glycosylation in Proteins
299
High Energy CollisionInduced Dissociation
321
HighPerformance Liquid Chromatographic Mapping
443
Monosaccharide Analysis of Recombinant Glycoproteins
457
Characterization of Subnanomolar Amounts of TVGlycans
491
Isolation and Characterization of TVLinked Oligosaccharides
509
Plant Glycobiology
533
Recent Developments in Starch Structure Analysis Using
553
Methodology for the Structural
567
Some New Methods to Study Plant Polyuronic Acids
595
Index
629
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