Adult Stem Cells
Academic Press, Dec 11, 2006 - Technology & Engineering - 576 pages
This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.
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activity adult airway antibodies apoptosis assay BD Biosciences beta cells Biol biology BMSCs bone marrow BrdU Brinster Bukovsky bulge cell lines cell population cell suspension cell types clones collagen colonies cord blood crypt derived differentiation dish Edited embryonic endothelial endothelial progenitor cells engraftment epidermis epithelial cells epithelium expression feeder fetal fibroblast follicular renewal function g/ml gene genetic germ cells growth factor hair follicle hematopoietic stem cells HSCs human hematopoietic incubated injection integrin intestinal Invitrogen isolated labeling lineage long‐term LRCs lung mammalian markers medium membrane mesenchymal mice mouse multipotent murine Natl neurons neurospheres NOD/SCID NSCs oocytes oogenesis ovarian ovaries phenotype plates pluripotent postnatal potential primary follicles progenitor cells proliferation protein protocol receptor regeneration regulation resuspended retinal self‐renewal serum signaling skeletal stem cells spermatogenesis SSCs staining sterile stromal cells studies tracheal transcription factor transgenic transplantation tube tubules vitro vivo VOLUME