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abnormalities acrosomal agglutination Aitken Amelar androgen antibodies antigens antisperm antibodies assessment associated azoospermia azoospermic bilateral biopsy cervical mucus chromosome Clin Endocrinol Metab cryptorchidism deferens detected donor effect endocrine epididymis erection Fertil Steril fertilisation FSH levels function germ cells germinal epithelium give details gonadotrophin hamster oocytes histological hormone human spermatozoa husband hypogonadism infertile couple infertility infertility clinic investigation Klinefelter's syndrome lesions Leydig cells male infertility meiotic membrane million/ml NO–O NO—O YES—O NO—O normal oligozoospermia patients penetration test penis pituitary pregnancy problems prolactin prostate reported reproductive retrograde ejaculation scrotal semen analysis semen sample seminal plasma seminiferous tubules Sertoli cells serum sexual sperm count sperm density sperm motility spermatogenesis spermatogenic spermatogonia spermatozoa steroid stimulation subfertile Table technique temperature testicular biopsy testis testosterone therapy thermography tissue titres treatment tubular Urol varicocele vasectomy vitro vitro fertilisation YES please give YES–O If YES YES–O NO—O YES–O zona-free hamster