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Biology of the Cultured Cell
Design and Layout of the Laboratory
Equipping the Laboratory
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Culture of Animal Cells: A Manual of Basic Technique and Specialized ...
R. Ian Freshney
Limited preview - 2011
acid agar ampules antibody assay autoclave autoradiography bottle Cancer caps cell concentration cell counter cell culture cell density cell lines cell proliferation cell strain cell suspension cell types centrifuge Chapter chromosome cloning collagen collagenase colonies containing continuous cell lines counting coverslips culture flasks cultured cells deionized deionized water differentiation dilute disaggregation embryo enzyme epithelial cells explant feeder layer fibroblasts filter filtration fluorescent freezer Freshney GIBCO glass glassware gradient growth medium hemocytometer hormones human incubator laboratory laminar flow laminar flow cabinets liquid medium methanol method microscope microtitration monolayer monolayer cultures mouse mycoplasma normal number of cells Pasteur pipette PBSA petri dishes phase pipette plastic plating efficiency preparation primary culture protein Protocol remove rinse sample Sato sedimentation seeded selective serum slide stain sterile stirrer storage subculture substrate surface technique tion tissue culture transformed trypsin tube tumor cells vitro vivo washing