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50 per cent adhesive resin Amer apex became carious Buonocore caries incidence caries prevention cent phosphoric acid clinical Clostridium histolyticum commercial occlusal sealant contaminated Control and Experimental control teeth cotton pellet cyanoacrylate Dent dental caries dental restorations eight day period enamel surface epoxy putty epoxy resin etching ethylene oxide evaluated evidence of bacterial exhibited bacterial penetration exhibited some evidence experimental design extracted teeth fissure caries fissure sealant glycidyl methacrylate gram stain Gwinnett investigation isotope marginal penetration methyl methacrylate methyl-2-cyanoacrylate microorganisms millimeters monomer Nuva-Seal observed occlusal caries occlusal opening occlusal surfaces Occlusal view paper point permanent molars permanent teeth petri dishes petri plate phosphoric acid physiologic saline Pierce 70 pit and fissure placement prepared tooth prevented bacterial penetration pulp chamber quadrant Ripa samples exhibited bacterial Sealant on teeth sealing of pits secured by epoxy silver nitrate Streptococcus mutans teeth Sealant test tube treated teeth trypticase soy broth utilized view of prepared