Studies of the Effects of the Chemical Modification of the Rifamycins on Their Interaction with DNA-dependent RNA Polymerase |
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Contents
rifamycin DNAdependent RNA polymerase | 8 |
The Spin Labelling Technique | 18 |
References | 36 |
15 other sections not shown
Common terms and phrases
3-amino 3-bromo rifamycin 3-cyano rifamycin 3-nitroxide rifamycin 3-substituted rifamycin acetone acid activity actual added addition angle ansa bridge antibiotics applied assay binding Biol buffer calculated changes Chem chemical shift chromatography column complex compound concentration conformation correlation coupling constants demonstrated dependent determined differences Dilution DNA-dependent RNA polymerase donor dried effect electron eluted enzyme equation ether ethyl acetate Figure filter fractions fragmentation function groups increase Inhibition Inhibitor Concentration initial interaction isolated linear lower mass mercapto method mg/ml minutes molecular nitroxide observed obtained occurring organic peak prepared protein protons ratio reaction removed reverse rifampicin rifamycin S derivatives rifamycin SV RNA polymerase sample silica gel similar solution solvent spectra spectrum spin label structure studies substituted SV derivatives synthesis Table tion Tris tubes volume washed yield