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100 cm suction aliquots bean seed capable chlamydospores colonization damping-off debaryanum decrease determined evacuated germ tube field capacity field soil fungal germ tube growth germination in soil germination occurred germlings glucose gram growth rate host exudations hr after amending hr after sowing hr at 2k hyphal hyphal strands incubation at 2k intercalary sporangia lysis ml sterile distilled moist soil mycelial naturally infested soil nutrient nutrient levels observed oospores parent sporangium pathogen percentage germination Permanent wilting point petri plates propagule protoplasmic Pythium spp Pythium ultimum Pythium ultimum Trow radial radicle rhizoplane rolled oat root exudates secondary sporangia seed coat exudate seed exudate seed surface seedlings septa septum soil amended soil dilution soil moisture soil samples soil smears spermosphere sporangial germination spore germination spores sterile distilled water substrates survival in soil survival mechanisms survival structures U8 hr ug glucose/g soil ultimum isolates ultimum population water agar zone of influence