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150 transfers 2,3-butylene glycol Acetic acid acetone and butyl Acetone Butyl acid and 2,3-butylene aliquot portion anaerobes basal medium biotin and p-aminobenzoic Biotin B.M. biotin medium butyl alcohol butyl alcohol-acetone bacteria butyl and ethyl Butyric acid Congo red Cultured on High-Test determination of acetone distillate ethyl alcohols Fermentations were carried gave good solvent Glucose and Biotin glucose-biotin medium glucose-molasses culture glycol were determined Growth Factor Requirements growth requirements high test cane High-Test Molasses inoculating Lactic acid M. N. MICKELSON media medium containing Medium Products Medium when Cultured molasses and synthetic needed both biotin nicotinamide p-aminobenzoic acid PABA Peterson produce solvents production of solvents pyridoxin required only biotin Requirements of Saccharolytic Residual sugar REYES-TEODORO AND M. N. riboflavin saccharolytic butyl alcohol-acetone solvent yields Solvents Produced Speakman's salts sterile subnormal yield sulfuric acid synthetic medium Table test cane molasses Thiamine three organisms Titration tothenic acid volatile acids Werkman yield of solvents