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0.1 millimicrogram added agar anhydrous Asparagine assay standard assay tubes autoclaved at 120 B12 standard solution B12 stock solution basal medium Beckman Model beef biotin buffer-bisulfite extractant concentration condensed milk Cystine diluted to 200 diluted with solvent dispensed in triplicate dissolved in distilled distilled water ethyl alcohol evaporated milk gram hydrochloride lated blanks leichmannii method malhamensis method malhamensis values mannii method materials were dissolved medium was stored millimicrogram per milliliter minutes moisture optical density organ meats overlaid with toluene p-aminobenzoic acid pH was adjusted provisional mean Salt solution sample extract screwcap polyethylene bottle Sodium sodium metabisulfite solution 20 ml solution was diluted solution was overlaid solution.—1 solvent to 200 spectrophotometer standard curve stock culture stored at 18 subsample replicates suspension medium tion of vitamin toluene and stored Tween 80 solution uninoculated blanks vitamin B12 content vitamin B12 standard Vitamin B12 stock Vitamin solution water and diluted Xanthine yoghurt