Gene Cloning: An Introduction |
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Page 62
... cleaved , but ( b ) bacterial DNA is not . ( a ) Restriction of phage DNA Phage injects DNA into a bacterium ( b ) ... cleave DNA at the recognition sequence and nowhere else . For example , the restric- tion endonuclease called Pvul ...
... cleaved , but ( b ) bacterial DNA is not . ( a ) Restriction of phage DNA Phage injects DNA into a bacterium ( b ) ... cleave DNA at the recognition sequence and nowhere else . For example , the restric- tion endonuclease called Pvul ...
Page 67
... cleave the DNA . The final ingredients in the reaction mixture are therefore 0.5 μl BglII + 1.5 μl water , giving a ... Cleaved λ DNA or heat at 70 ° C for 15 minutes Table 4.2 A 10 x buffer suitable for restriction of Restriction ...
... cleave the DNA . The final ingredients in the reaction mixture are therefore 0.5 μl BglII + 1.5 μl water , giving a ... Cleaved λ DNA or heat at 70 ° C for 15 minutes Table 4.2 A 10 x buffer suitable for restriction of Restriction ...
Page 80
... cleaves the chains at the recogni- tion sequences , producing a large number of cleaved linkers and the original DNA ... cleave the blunt - ended molecule ( Figure 4.22 ) . The resulting fragments will have the correct sticky ends , but ...
... cleaves the chains at the recogni- tion sequences , producing a large number of cleaved linkers and the original DNA ... cleave the blunt - ended molecule ( Figure 4.22 ) . The resulting fragments will have the correct sticky ends , but ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast