Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-6
... Ampicillin binds to and inhibits a number of enzymes in the bacterial membrane that are involved in the synthesis of the cell wall ( for review , see Waxman and Strominger 1983 ) . The ampicillin resistance ( amp1 ) gene carried on the ...
... Ampicillin binds to and inhibits a number of enzymes in the bacterial membrane that are involved in the synthesis of the cell wall ( for review , see Waxman and Strominger 1983 ) . The ampicillin resistance ( amp1 ) gene carried on the ...
Page 1-81
... ampicillin is selected , only a portion of the culture ( empirically determined ) should be spread on a single plate . The number of ampicillin - resistant colonies does not increase in linear proportion to the number of cells applied ...
... ampicillin is selected , only a portion of the culture ( empirically determined ) should be spread on a single plate . The number of ampicillin - resistant colonies does not increase in linear proportion to the number of cells applied ...
Page 1-87
... ampicillin ) . Some of the colonies that grow in the presence of ampicillin will contain recombinant plasmids ; others will contain plasmid DNA that has recircularized during ligation without insertion of the foreign DNA . To ...
... ampicillin ) . Some of the colonies that grow in the presence of ampicillin will contain recombinant plasmids ; others will contain plasmid DNA that has recircularized during ligation without insertion of the foreign DNA . To ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml