Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 41
Page 1-78
... Final concentration 10 mм 10 ml 8.91 g 45 mM 10 mM 1.47 g 7.46 g 100 mM 0.80 g 3 mм 2 1 м MES ( 2- [ N - morpholino ] ethanesulfonic acid ; Ferguson et al . 1980 ) is prepared by dissolving 19.52 g of MES ( Good and Izawa 1972 ) in 80 ...
... Final concentration 10 mм 10 ml 8.91 g 45 mM 10 mM 1.47 g 7.46 g 100 mM 0.80 g 3 mм 2 1 м MES ( 2- [ N - morpholino ] ethanesulfonic acid ; Ferguson et al . 1980 ) is prepared by dissolving 19.52 g of MES ( Good and Izawa 1972 ) in 80 ...
Page 2-80
... final concentration of 20 mM . 5. Add pronase ( self - digested ) to a final concentration of 0.5 mg / ml , or add proteinase K to a final concentration of 50 μg / ml . Self - digested pronase is prepared by dissolving powdered pronase ...
... final concentration of 20 mM . 5. Add pronase ( self - digested ) to a final concentration of 0.5 mg / ml , or add proteinase K to a final concentration of 50 μg / ml . Self - digested pronase is prepared by dissolving powdered pronase ...
Page 7-7
... final concentration of 200 μg / ml . Mix the solution well and incubate for 30 minutes at 37 ° C . Proteinase K is stored as a stock solution at a concentration of 20 mg / ml in water ( see Appendix B ) . Lysis of cells growing in ...
... final concentration of 200 μg / ml . Mix the solution well and incubate for 30 minutes at 37 ° C . Proteinase K is stored as a stock solution at a concentration of 20 mg / ml in water ( see Appendix B ) . Lysis of cells growing in ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml