Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 63
Page xxiv
... DNA by Southern Hybridization 9.31 SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 ...
... DNA by Southern Hybridization 9.31 SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 ...
Page 3-2
... genomic DNA . As discussed in Chapter 9 , the number of recombi- nant clones required to form a complete library of genomic DNA is fixed not only by the size of the genome , but also by the capacity of the vector : The larger the fragment ...
... genomic DNA . As discussed in Chapter 9 , the number of recombi- nant clones required to form a complete library of genomic DNA is fixed not only by the size of the genome , but also by the capacity of the vector : The larger the fragment ...
Page 5-50
... genomic DNA or single- stranded cDNA as the original template , can result in a 10 - fold amplification of specific sequences . It is therefore extremely useful for ... DNA Polymerase (Sequenase TM) 5 49 Taq DNA Polymerase and AmpliTaq 5 50.
... genomic DNA or single- stranded cDNA as the original template , can result in a 10 - fold amplification of specific sequences . It is therefore extremely useful for ... DNA Polymerase (Sequenase TM) 5 49 Taq DNA Polymerase and AmpliTaq 5 50.
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
47 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml