Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 32
Page 1-92
... filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C until the results of the screening procedure become available ... Nitrocellulose Filters 1.
... filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C until the results of the screening procedure become available ... Nitrocellulose Filters 1.
Page 1-94
... nitrocellulose filter and lay it on the master nitrocellulose filter . Take care to prevent air bubbles from becoming trapped between the two filters . This is best done by bending the second filter slightly so that contact is first ...
... nitrocellulose filter and lay it on the master nitrocellulose filter . Take care to prevent air bubbles from becoming trapped between the two filters . This is best done by bending the second filter slightly so that contact is first ...
Page 2-112
... filter prior to hybridization . Because more bacteriophage DNA becomes attached to the nitrocellulose filter , the au- toradiographic signals from positive clones are enhanced ... Nitrocellulose Filters Following Situ Amplification 2.
... filter prior to hybridization . Because more bacteriophage DNA becomes attached to the nitrocellulose filter , the au- toradiographic signals from positive clones are enhanced ... Nitrocellulose Filters Following Situ Amplification 2.
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml