Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-93
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Replicating Colonies onto Nitrocellulose Filters Two methods are given here for replicating colonies onto nitrocellulose filters . Method 1 ( Hanahan and Meselson 1980 ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Replicating Colonies onto Nitrocellulose Filters Two methods are given here for replicating colonies onto nitrocellulose filters . Method 1 ( Hanahan and Meselson 1980 ...
Page 1-94
... nitrocellulose filter off the storage plate and lay it , colony side up , on a dampened pad of sterile 3MM paper . c . Number a damp , sterile nitrocellulose ... filters . f . Peel the filters apart . Lay the replica 1.94 Plasmid Vectors.
... nitrocellulose filter off the storage plate and lay it , colony side up , on a dampened pad of sterile 3MM paper . c . Number a damp , sterile nitrocellulose ... filters . f . Peel the filters apart . Lay the replica 1.94 Plasmid Vectors.
Page 2-109
... Nitrocellulose Filters or Nylon Membranes This method is modified from that of Benton and Davis ( 1977 ) . 1. Mix aliquots of a packaging reaction or bacteriophage A stock containing no more than 50,000 bacteriophage particles in a ...
... Nitrocellulose Filters or Nylon Membranes This method is modified from that of Benton and Davis ( 1977 ) . 1. Mix aliquots of a packaging reaction or bacteriophage A stock containing no more than 50,000 bacteriophage particles in a ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml