Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 54
Page 2-59
... recA HB101 гв mв supE supF hsdS DP50supF гв mg Rec supE supF hsdS recA ED8767 C strain C - 1a Permissive for vectors carrying amber mutations Will modify but not restrict DNA Permits a complementation of B - galactosidase Will modify ...
... recA HB101 гв mв supE supF hsdS DP50supF гв mg Rec supE supF hsdS recA ED8767 C strain C - 1a Permissive for vectors carrying amber mutations Will modify but not restrict DNA Permits a complementation of B - galactosidase Will modify ...
Page 2-96
... recA function in the lysogens can be tested as follows : i . Streak the strain being tested , a known recA strain , and a wild - type E. coli strain onto a plate as shown in Figure 2.7 ( top ) . ii . Use a piece of cardboard to cover ...
... recA function in the lysogens can be tested as follows : i . Streak the strain being tested , a known recA strain , and a wild - type E. coli strain onto a plate as shown in Figure 2.7 ( top ) . ii . Use a piece of cardboard to cover ...
Page 5-88
... RecA - coated single - stranded DNA Double - stranded target DNA FIGURE 5.5 Initial homologous pairing D - loop The use of RecA protein to produce a displacement loop in double - stranded DNA . Topoisomerase I ( Calf thymus ) ...
... RecA - coated single - stranded DNA Double - stranded target DNA FIGURE 5.5 Initial homologous pairing D - loop The use of RecA protein to produce a displacement loop in double - stranded DNA . Topoisomerase I ( Calf thymus ) ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml