Bioseparations engineering: principles, practice, and economics
Multidisciplinary resource for graduate studies and the biotechnology industry
Knowledge of the genetic basis of biological functioning continues to grow at an astronomical rate, as do the challenges and opportunities of applying this information to the production of therapeutic compounds, specialty biochemicals, functional food ingredients, environmentally friendly biocatalysts, and new bioproducts from renewable resources. While genetic engineering of living organisms transforms the science of genomics into treatments for cancer, diabetes, and heart disease, or products for industry and agriculture, the science and technology of bioseparations are the keys to delivering these products in a purified form suitable for use by people.
The methods, theory, and materials that reduce the science of bioseparations to practice, whether in the laboratory or the plant, are the subjects of Bioseparations Engineering. Examples address purification of biomolecules ranging from recombinant proteins to gene therapy products, with footnotes detailing economics of the products. Mechanistic analysis and engineering design methods are given for:
* Isocratic and gradient chromatography
* Sedimentation, centrifugation, and filtration
* Membrane systems
* Precipitation and crystallization
Topics addressed within this framework are: stationary phase selection; separations development; modeling of ion exchange, size exclusion, reversed phase, hydrophobic interaction, and affinity chromatography; the impact of regulatory issues on chromatography process design; organization of separation strategies into logical sequences of purification steps; and bridges between molecular biology, combinatorial methods, and separations science.
A result of teaching and developing the subject matter over ten years, Bioseparations Engineering is an ideal text for graduate students, as well as a timely desk book for process engineers, process scientists, researchers, and research associates in the pharmaceutical, food, and life sciences industries.
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SEDIMENTATION CENTRIFUGATION AND FILTRATION
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acetonitrile adsorbent adsorption affinity chromatography amino acid binding Bioseparations buffer calculated cells cellulose centrifuge Chem chemical chromatography column column length component constant crystallization curve decrease diameter distribution coefficient elution profile enzyme equation equilibrium ethanol feed fermentation filter filtration flowrate fluid flux function given gradient hydrophobic illustrated in Figure immobilized injected insulin interstitial velocity ion exchange ion exchange chromatography ionic strength isotherm ligand linear liquid chromatography lysozyme M. R. Ladisch mass transfer membrane microfiltration micron mobile phase molecular weight molecules NaCl nucleation packed packed bed particle peak peptide permission plate count plate height pressure drop protein Protein Purification purification ratio recombinant resin reversed phase salt concentration scale scale-up Schematic separation sequence shown in Figure size exclusion chromatography solid solubility solute solvent stationary phase step surface Table temperature Velayudhan void fraction void volume width