Manipulating the Mouse Embryo: A Laboratory ManualRegarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual,the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting–edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitrofertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields. Key features of the third edition: New authors are leading authorities in mouse development and genetics, as were authors of prior editions. All new illustration and new design: All illustrations have been redrawn; extensive use of new illustrations and photographs of techniques and results; a new, contemporary design presents techniques more clearly. New explanatory text: More background information for techniques is presented as well as more extensive troubleshooting information. New, functional organization and techniques. |
Contents
Summary of Mouse Development | 31 |
General Issues | 141 |
Recovery and In Vitro Culture of Preimplantationstage Embryos | 161 |
Copyright | |
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Common terms and phrases
acid allele animals appropriate handling Biol blastocyst Brinster buffer capillary cell lines Chapter chimeras chromosome cloning colonies containing Cre recombinase cryopreservation culture medium developmental differentiation dissection ectoderm EDTA embryo culture embryo transfer embryonic stem cells endoderm epiblast ethanol extraembryonic females fertilization filter forceps gastrulation genetic genome germ cells glass gloves and safety handling of materials holding pipette hybridization inbred incubator injection pipette KSOM laboratory M2 medium male mammalian materials marked membrane mesoderm method microinjection micromanipulation microscope minutes mouse mouse embryos mutation Natl neural oocytes ovary oviduct Pasteur pipette plasmid plate postimplantation preimplantation primitive streak procedure pronucleus protein Protocol pseudopregnant REAGENTS recombinase recombination remove Reprod room temperature serum Sigma slides solution somites sperm stage sterile strains superovulated targeted tion tissue culture dish transgenic transgenic mice tube uterus visceral vitro Wear appropriate gloves yolk sac zona pellucida zygotes