The Chromatography of Steroids: International Series of Monographs on Pure and Applied Biology: BiochemistryThe Chromatography of Steroids details the fundamental concepts and underlying principles of laboratory techniques utilized in separating steroid mixtures. The text first covers the basic theory of chromatography, and then proceeds to tackling the chromatographic separation of steroids. Next, the selection details the techniques and apparatus employed in chromatography of steroids. Chapter IV talks about the colorimetric and radioisotopic techniques, while Chapter V deals with the structural analysis and identification of steroids by chromatography. The text also coves the typical analytical problems of steroid biochemistry. The book will be of great use to researchers who utilizes chromatographic methods in their work with steroids. |
Contents
| 1 | |
CHAPTER II CHROMATOGRAPHIC SEPARATION OF STEROIDS | 33 |
CHAPTER III TECHNIQUES AND APPARATUS | 133 |
COLORIMETRIC AND RADIOISOTOPIC TECHNIQUES | 201 |
CHAPTER V STRUCTURAL ANALYSIS AND IDENTIFICATION OF STEROIDS BY CHROMATOGRAPHY | 256 |
CHAPTER VI SOME TYPICAL ANALYTICAL PROBLEMS OF STEROID BIOCHEMISTRY | 311 |
APPENDICES | 347 |
REFERENCES | 389 |
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Common terms and phrases
17-oxosteroids acetic acid acetylation adsorbent adsorption alcohols aldosterone alkaline alumina aqueous methanol systems ARMg values ARMr benzene Biochem biol Bush and Mahesh Bush and Willoughby Celite cellulose changes Chem chromato chromatogram chromic acid colour column compounds concentration conjugates cortisol derivatives diacetate eluate elution epimeric equilibration esters estimation ether ethyl acetate evaporation extract filter paper fluorescence formamide fraction give glucuronosides hydrocarbon hydroxyl groups impregnation interactions ketone ketone groups large number less polar Lewbart Martin material methanol method mixture mobile phase modifying step molecules NaOH obtained oestrogens oxidation paper chromatograms Paper imp polar solvent probably procedure progesterone propylene glycol quantitative reagent Reichstein Reineke reversed-phase RF values scanning separation silica gel sodium solubility solution solvent systems specific stationary phase steric steroids strip substance substituents sulphate t-butanol Tait technique tetrahydrocortisol toluene typical unpublished urine usually volatile washed Zaffaroni zone