Fractionation of the Acetylated Forms of the Core Histones and the Effects of Acetylation in Reconstituted NucleosomesU. of Calif., Davis, 1990 - 278 pagini |
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Isolation and Characterization of Acetylated | 14 |
Summary | 17 |
Results | 21 |
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Termeni și expresii frecvente
Acad acetate acetylated forms acetylated histones acetylated nucleosome acetylation of H3 Acid Res acid urea gel acrylamide Allfrey amino acid bands Biochem Biochemistry Biol Biophys Bradbury butyrate treated cation exchange Chalkley change per nucleosome Chem chromatin chromatogram CM-HPLC control core control histones cysteines denatured dialyzed digestion DNase EDTA ethanol exchange HPLC forms of H3 four core histones fractionated fully acetylated H3 H2A and H2B H3 and H4 highly acetylated histone acetylation histone H3 histone octamer HPLC Imai intermediately acetylated iodoacetamide ion exchange isolation labelled Lane levels of acetylation linking number change lysine lysine 27 lysine 9 method micrococcal nuclease minichromosomes minichromosomes reconstituted mononucleosomal N-terminal N-terminal domains NaCl Natl non-coding strand nuclei nucleoprotein nucleoprotein particle gels nucleosome core particle nucleotides phasing sequence Physarum polycephalum PMSF Proc procedure purified Reconstituted Nucleosomal Particles RP-HPLC samples sites of acetylation sodium butyrate supercoiling thiol treated HeLa cells Tris-HCl unacetylated urea variants