Essential Zebrafish Methods: Genetics and Genomics

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H. William Detrich, III, Leonard I. Zon, Monte Westerfield
Academic Press, Sep 5, 2009 - Technology & Engineering - 400 pages
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Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab.



    * Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors * Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error

     

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    Contents

    Haploid Screens and GammaRay Mutagenesis
    1
    Positional Cloning of Mutated Zebrafish Genes
    29
    The Zebrafish Genome
    47
    RetroviralMediated Insertional Mutagenesis in Zebrafish
    61
    Genetic Screens for MaternalEffect Mutations
    81
    Behavioral Screening Assays in Zebrafish
    113
    TILLING the Zebrafish Genome A Reverse Genetics Approach
    131
    The Transgenesis and Gene and Enhancer Trap Methods in Zebrafish by Using the Tol2 Transposable Element
    153
    Transgene Manipulation in Zebrafish by Using Recombinases
    233
    Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease ISceI
    255
    Cloning Zebrafish by Nuclear Transfer
    277
    Spatial and Temporal Expression of the Zebrafish Genome by LargeScale In Situ Hybridization Screening
    287
    Genetic Backgrounds Standard Lines and Their Husbandry
    303
    Common Diseases of Laboratory Zebrafish
    321
    Zebrafish Sperm Cryopreservation
    347
    Index
    365

    Molecular Cytogenetic Methodologies and a SecondGeneration BAC Probe Panel Resource for Zebrafish Genomic Analyses
    175
    The Zon Laboratory Guide to Positional Cloning in Zebrafish
    191
    Sleeping Beauty Transposon for Efficient Gene Delivery
    219
    Color Plates
    375
    Copyright

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    About the author (2009)

    Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

    Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

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