Handbook of Basic Microtechnique |
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Page 80
... thickness of approximately 1 mm is the optimum , but slides as thick as 14 mm are perfectly satisfactory . It is essential that the sur- face of the slide be flat and that the glass of which it is composed be as stable as possible , for ...
... thickness of approximately 1 mm is the optimum , but slides as thick as 14 mm are perfectly satisfactory . It is essential that the sur- face of the slide be flat and that the glass of which it is composed be as stable as possible , for ...
Page 130
... thickness , to accommodate objects of varying size . This method , however , is not satisfactory for objects much more than 0.5 mm thick because the wide rim left at the edge of the cover causes the mountant to dry out very rapidly . An ...
... thickness , to accommodate objects of varying size . This method , however , is not satisfactory for objects much more than 0.5 mm thick because the wide rim left at the edge of the cover causes the mountant to dry out very rapidly . An ...
Page 139
... thick when cold , so that the coverslip will not become displaced if the mount is handled before the balsam is fully hard . The hardening may be aided by heat , but care must be exer- cised in heating thick mounts , particularly where ...
... thick when cold , so that the coverslip will not become displaced if the mount is handled before the balsam is fully hard . The hardening may be aided by heat , but care must be exer- cised in heating thick mounts , particularly where ...
Contents
Principles of Microscopy | 3 |
The Use of the Microscope | 31 |
Photomicrography | 55 |
Copyright | |
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70 per cent absolute alcohol acid agent angle animal appear balsam become better block blue cause cell cent alcohol Chapter clean clear color completely containing coverslip cytoplasm dehydrating described desired differentiation drop edge embedding embryo employed examined example field filter fluid given glass green hand hardened hematoxylin illumination iris knife known lamp least leave lens less light manner material medium method microscope microtome mixture mounted necessary nuclei object obtained operation paraffin sections passed placed position possible prefer preparation preserved produce Recommended relatively remain removed result ribbon Rinse seen selected shown in Fig shows side slide smear solution specimen stage stain staining solution Structure substage sufficiently surface taken technique thick tissues transferred tube usually washed waves whole wholemounts xylene